Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLSIGLIIMLSLFGCTDSKAKNQEKFLTHIHNNTPEPYKECMVMYIKNHWDDVWKTYDSEKVREARGETDIVNFMIERFLPECKK 1N8V Chain:B ((57-95)) ---------------KCTENQEKGAYRVIEHLIKNEI------------EIWRELTAKYDPTGNWR---------------------

General information: TITO was launched using: RESULT: Template: 1N8V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 49 -3018 -61.59 -77.38 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : -61.59 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.623

(partial model without unconserved sides chains): PDB file : Tito_1N8V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N8V-query.scw PDB file : Tito_Scwrl_1N8V.pdb: