Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKCFSHIRREEPMMIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVIDFFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIEWTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIKRHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIP----PIDSYIITPELGDNAGIIGGLALARKAVRNKQP 1XC3 Chain:A ((4-292)) --------------MLGGIEAGGTKFVCAVGREDGTIIDRIEFPTKMPDETIEKVIQYFSQFS--LQAIGIGSFGPVDNDKTSQTYGTITATPKAGWRHYPFLQTVKNEMKIPVGFSTDVNAAALGEFLFGEAKGLDSCLYITIGTGIGAGAIVEGRLLQGLSHPEMGHIYIRRHPDDVYQGKCPYHGDCFEGLASGPAIEARWGKKAADLSDIAQVWELEGYYIAQALAQYILILAPKKIILGGGVMQQKQVFSYIYQYVPKIMNSYLDFSELSDDISDYIVPPRLGSNAGIIGTLVLAHQALQ----

General information: TITO was launched using: RESULT: Template: 1XC3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1691 -182196 -107.74 -639.28 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -107.74 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_1XC3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1XC3-query.scw PDB file : Tito_Scwrl_1XC3.pdb: