Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEENKMAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPAIKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVKVGMMGPLAGVGDPVFWFTVRP-MLGALGASLAMGGNILGPIIFFLG-WNIIRWAFMWYTQEFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKGAYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMWLLKKKVSPIVIILGLFVVGVVGHVIGLL 3ME1 Chain:A ((42-130)) ----------------------------------------------------------------------FYNSTTLGVCAVLGTAASTLTAQLLG--VDKPLVRAGLFGFNGTLAGIALP-FFFNYEPAMLGYVALNGAFTTIIMASLLNFLGKWGV----------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ME1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 0 0 NaN 0.00 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : NaN 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.54 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_3ME1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ME1-query.scw PDB file : Tito_Scwrl_3ME1.pdb: