Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGLAVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE 3H46 Chain:X ((396-498)) MKKDSGIDIPLLKVDGGAAKNDLLMQFQADILDIDVQRAANLETTALGAAYLAGLAVGFWKDLDELKSMAEEGQMFTPEMPAEERDNLYEGWKQAVAATQTFK

General information: TITO was launched using: RESULT: Template: 3H46.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 221 -16286 -73.69 -158.12 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain X : 0.95 3D Compatibility (PKB) : -73.69 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_3H46.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H46-query.scw PDB file : Tito_Scwrl_3H46.pdb: