Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVIEKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGGCFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYEELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPIVTEIRQAKTFWPAENHHQQFYQKQ-PKRYKKIKHSHQLHLFFQRHR-------------------------------------------- 4LWL Chain:A ((6-210)) --------------------------------------------------------------------------------------------KLSIAVFALGCFWGPDAQFGSIKGVVSTRVGYAGGTTNNPSYYNLGD----HSASIEIQYDANVITYGELLNIFWNLHNPVY-----ETTNRQYMSRIFYLDDGQKSEALEMKRQIEAANG--EKIYTEIVPLENFYLAEGYHQKYYLQNTTKLYQTLKAIYGGFGNLVRSTLAARMNGYIAGNLSIASLKEEMDLVELPEDQYEKVLSIVEEIKL

General information: TITO was launched using: RESULT: Template: 4LWL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 731 -53723 -73.49 -335.77 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -73.49 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_4LWL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LWL-query.scw PDB file : Tito_Scwrl_4LWL.pdb: