Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAISTKELMEVTLGKKLGAGSFGSVFVGV-LPSGNFVAVKILELSDDAPSN---TEVEIHRKMVHPNIIRYLYSRIDLESTPKKLYVYLEFVTGGSVTSLMKSLPNGCLPYAVVRVYARHMFQGLEYLHSNQVAHRDIKGDNVLISMDTGTAKLADFDQAKIMNTHGTLRKAATATLAGTPYWMAPEVITDEDGYDPFKADIWSAGCTVAEMITGRAPWTPMPNVMHIMNKLALSTGWPDAVPKDAKELGSQDAYDFLDLCFQRDVSKRPPAATLLKHVFLRV 4EQC Chain:A ((30-279)) ---------------EKIGQGASGTVYTAMDVATGQEVAIRQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYL----VGDELWVVMEYLAGGSLTDVVTET---CMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMD-GSVKLTDFG----FCAQITPEQSKRSTMVGTPYWMAPEVVT-RKAYGP-KVDIWSLGIMAIEMIEGEPPYLN-ENPLRALYLIA-TNGTPEL--QNPEKL-SAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKI

General information: TITO was launched using: RESULT: Template: 4EQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1176 -36261 -30.83 -147.40 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -30.83 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_4EQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EQC-query.scw PDB file : Tito_Scwrl_4EQC.pdb: