Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSARRSRLRRVRWLPHQRIVVLKEVDLWAEDCVTEGVDVVAERLWRMRMDASRNGRAQYLAEYLALHVDSSRHYQWLLSNY-GPSLRDVLLTSSLTPFTEERCKGVLRRVLLALDQLHRVSMYVHADVSLGNVLTSPSSCDDVVLGDLESVSPIGAVP-RGCLGSYLFMAPERLVDG-ALPLAPHDDIWAFGIVCYNLLTWDVTHPWCALGTESDHSENYWAFLDLMSKAKDVPPCQLLLQSPLSRCSKEALTFVAVCLSWDPACRPSAAELLQHSWMLSP 5J5T Chain:A ((13-267)) GSGTYGDVYKARNVNTGELAAIKVIKLEPGE-DF---AVVQQEIIMMKDC--K---HPNIVAYFGSYLRRDKLW-ICMEFCGGGSLQDIYHVT--GPLSELQIAYVSRETLQGLYYLHSK-GKMHRDIKGANILLTDNGH--VKLADFGVSAQITATIA-AFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQ--PPMFDLHP--------MRALFLMTKSNFQPP-KL---KDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQ-

General information: TITO was launched using: RESULT: Template: 5J5T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1084 -73291 -67.61 -296.72 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -67.61 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_5J5T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J5T-query.scw PDB file : Tito_Scwrl_5J5T.pdb: