Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSTSVALIASVLRQPRVGDDLQWVRYDAPFRTAQDYAAAEYLYRLRLCSLRLRSSLLSPSLPRTGPVSEVAHLLPHYPVQITAQLVYMDEPLSPVVESAAVYASAAALLPPASPVGGDTDTHAEMEVVYTFTDEWLVFPLELCDVPLDASCKLHLWHRDTHVAEAALHVYSVAGEL--CVGQRQLALSTGGDAPSDEQLWSTSTRAAELLADFHRGMMPPIPWLDTLSIQQLEAEHARHGNAVVRRGSPQSAKDPCGAPTAILTLYLPAATTAV-FFEPGVARVSDDMKSLLQHSDAADDSCDFTQRPFPDQYTFFKEHNLCEAKAAITSKTQYFLSDSSAPPGPKERHQLASLLRRPPIQLDNVAGAVVTGAGAGGVGGGRLEETRLLWKYRHFICRDGKYFLPFMRCVDWANTHSSERRAACALIYQWARPAFEDVLACLSFYFDHVAPVRQYAVRLLRREGDGRLCQLAFQLVQAVRYDSAEAELANFLVERAVGCWELCSTLYTLLSVEVALEKRRTLSAAGKASDDRHGGRALFEPLLRRLSERLTQQCPHFATRLRQQHAMHRVLQLLSRQLQQSSLDRLGKTALGNKLIAKQACGLRALFSSVHHGMPSRRNVNGSFSSSLMTHSSAGMREEHSMSSARTGDGSEEADANGGDGSSAVEASDVDSAADDEVRRLRFPPLSPQSQGSAPRSPPSQSGAKQQYRSRHASAVDVLDRYGVATLATHPGIPITGLLSDSLYIFKSAKLPMRLTFTALRPAGLAWGGGRGGEPLYGASPSPCLLTPLAMQPAPSQQRSGGLQGMNSSGGGASAAVAAEEQAEGFLGAGEGDTVPLAMMYKYNDDIRQDQLIVQLIRLMDDLLQRDGLQLYLTPYRVIATAPNEGLVEIVP--QVTTFFSVQRDVLKYLRVY----NSTAELLRQAMDRYTRSFAGYCVITFVLGIGDRHLENILITQDGRLLHIDFGYVLGNDPKPFPPPMKINREMVEVLGGPQSTGFTEFKLYCCSAYNTLRKHAPLLLHILLLGAHTEGMPQVTGEGGDPRVNLLKVQEKLRLDLTNAQATQYLQNVIADSVGSIFTNLWDVLHAAAQATRG 5DFZ Chain:C ((4-875)) --------------------------NNITFCVSQDL---DVPLKVKIKSLEGHKPLLKPSQKILNP-----------ELMLIGSNVFPSSDLIVSLQVFDKERNRNLTLPIYTPYIPFRNSRTW--------DYWLTLPIRIKQLTFSSHLRIILWEYNGSKQIPFFNLETSIFNLKDCTLKRGFESLKFRYDVIDHCEVVTDNKDQENLNKYFQGEFTRLPWLDEITISKLRKQREN-------RTWPQG--------TFVLNLEFPMLELPVVFIEREIMNTNDPIEEKYRRLERASKNANL---------------------------------DKQVKPDIKKRDYLNKIINYPPGTKLT------------------AHEKGSIWKYRYYLMNNKKALTKLLQSTN--LREESERVEVLELMDSWAEIDIDDALELLGSTFKN-LSVRSYAVNRLKKASDKELELYLLQLVEAVCFEN--------------------------------------LSTFSDKSNSEFTIVDAVSPL--------------AEFLIRRALVNPRLGSFFYWYLKSESEDK-----------------------------PYLDQILSSFWSRLDKKSRNILNDQ-------------------------VRLINVLRECCETIKRLK----------------DTTAKKMELLVHLLETKVRPLVKVRPIALPLDPDVLICDVCPETSKVFKSSLSPLKITFK---------------------------------------------TTLNQ---------------------------PYHLMFKVGDDLRQDQLVVQIISLMNELLKNENVDLKLTPYKILATGPQEGAIEFIPNDTLASILSKYHGILGYLKLHYPDENATLGVQGWVLDNFVKSCAGYCVITYILGVGDRHLDNLLVTPDGHFFHADFGYILGQDPKPFPPLMKLPPQIIEAFGGAESSNYDKFRSYCFVAYSILRRNAGLILNLFELMKTSN----IPDIRIDPNGAILRVRERFNLNMSEEDATVHFQNLINDSVNALLPIVIDHLHNLAQYWRT

General information: TITO was launched using: RESULT: Template: 5DFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 3311 -61530 -18.58 -79.50 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -18.58 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_5DFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DFZ-query.scw PDB file : Tito_Scwrl_5DFZ.pdb: