Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLTSRTAQAAHAQNDVAAQRPPPPLYPSMNVGVSAVHTHQNRPCHPYDSQPAWQHPQLGTAPPAVIAGASLAPEQQEQAPQPQQPQQTHQQGRSNQPQSIFGGRFTLLDRLGSGGFGEVYRAEERDQLVPIAVKVERVTDTNALPEQSFLFHEAKVMQEIHKSIQAYMAAQQQHQLMLLQQEKARSGNGRRAENEAAAAADETRQEKVGIAKLKYYGQDGMSRVLIMSLHGQSVANVHRH-QGRLSLFATVMIADQVLRSLEHVHRAGYVHADLKPDNILFGRE-DPEQLYLVDFGLSVHFRDRKGK-HRPLITNHSFVGTPRYASLRTHMGHTLSRRDDIEQLVYVMIYLFRGRLPWSGLRISDPDAKEKRIAQMKAEMTLDSICAGCPEAFRDVLNYARCMEFEEEPQYQFLHVLLCSLRDSCTELSSDPNGVPANGSGGVMPQMLTLNANCSTRQHPKAQNGGDAVANGGAVCMTAAEAGLVKNTNIASGVVEQIDGVAENPMMMSSRGTGAPAFFSDAIGQGFLSGNGTDAGPLSPRIDCLNGQPFSPPPLDLQRPCGSPQLRQMR 5FQD Chain:F ((14-292)) --------------------------------------------------------------------------------------------------EFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKA-----RHPQLLYESKLYKILQG--------------------------------------------GVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTL-------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FQD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1361 -80560 -59.19 -291.88 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain F : 0.69 3D Compatibility (PKB) : -59.19 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.585

(partial model without unconserved sides chains): PDB file : Tito_5FQD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FQD-query.scw PDB file : Tito_Scwrl_5FQD.pdb: