Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRMNRNQEDSIQPESNKDVMVHARAQSVTKSVQHRPSPHENDVDGAADAPPRRAAGARSRSPPLPSAQQRGCTRVESTSCTYTLREEIGRGEYGRVFLADATPHQLQPQLPLQHSQSASFLPSPTSGVVVGLGSKGRSEPRNAGGRPSRMVLKRMDNTSVEDGLQAATLREIMVLDEVSAGTSNRDRVERDAFRRGRAVFRPATAAPSNTHVQEGFPSSFVDHNRYCSGGAAEDEWDDAALSSSGPLRIGALDALSAQQRRRISRGRQHLIGIRDAILRPREQLAYVVMDYCEGGDLWHFMRDLKVSVRSSSGKFGDIMSTRVFRRWSVELILALGFLHSHNIAHRDLKPQNLMLAKRTDAILSSSFAVDSANGSELCDIEGAPAGELYTLKVGDFGLSRLEGIPYKKYVHEAVTLWYRSPDVLLGNTNYTYSADAWSLGCILVEMASGTVLFKGKDEADELRHIFTRGCRPSPSIFPRMRDYHYCERYAEVLNRYQEADTENMTSEELMQMQLERISRHLRAFLRDRHALELLGDAGVDLVARLLILDPERRLTVLEAMNHRFFTNAYAEVYGADG 3GC0 Chain:A ((32-326)) ------------------------------------------------------------------------------SIDRYRRITKLGEGTYGEVYKAID------------------------------------------TVTNETVAIKRIRLEH-------TAIREVSLLKELQ---------------------------------------------------------------------------------------HRNIIELKSVIHH--NHRLHLIFEYAEN-DLKKYMDKNP------------DVSMRVIKSFLYQLINGVNFCHSRRCLHRDLKPQNLLLSV-----------------------------ETPVLKIGDFGLARAF---------EIITLWYRPPEILLGSRHYSTSVDIWSIACIWAEMLMKTPLFPGDSEIDQLFKIFEVLGLPDDTTWPGVTALPD---W---KQSFPK--------------FRGKT---LKRV-----LGALLDDEGLDLLTAMLEMDPVKRISAKNALEHPYFSHND--------

General information: TITO was launched using: RESULT: Template: 3GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1390 -193694 -139.35 -704.34 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -139.35 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_3GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GC0-query.scw PDB file : Tito_Scwrl_3GC0.pdb: