Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence TPMLDSLIEHRSPIFCLVQMCEMLDGGKKYCTR------WYKVYPGGSHTFQEGGRDT-ITVTFDNKCKKSLSGSTKKRSIGFPTQLVYRSDDPILAGISKIFEPGRAPNYH 5T80 Chain:H ((90-141)) ----------------------MEDSGLYFCARTGKYYDFWSGYPPGEEYFQDWGRGTLVTVS---------SASTKGPSV-FP----------------------------

General information: TITO was launched using: RESULT: Template: 5T80.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 74 2030 27.43 45.11 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain H : 0.63 3D Compatibility (PKB) : 27.43 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_5T80.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T80-query.scw PDB file : Tito_Scwrl_5T80.pdb: