Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----PYV---SERSVDLQTPAPINCVVVLRFRATDQMVR----GQ--------SRLVPAGDSTRFKLGNQSYLVFTGR-----DCTVVDPAAGGQLPAEYYFFGMPSPQI---DSD- 1FNG Chain:A ((82-182)) DANVAPEVTVLSRSPVNLGEPNILICFIDKFSPPVVN-VTWLRNGRPVTEGVSETVFLPRDDHLFRKF---HYLTFLPSTDDFYDCEVDHW-------------GLEEPLRKHWEFEE

General information: TITO was launched using: RESULT: Template: 1FNG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 243 14219 58.51 197.48 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 58.51 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_1FNG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FNG-query.scw PDB file : Tito_Scwrl_1FNG.pdb: