Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WDLLPSAEQVGKLVPSAEQIGKWVLSKEQIDALFFYQCQVTIHYPGAERGKLLKYLAEPREPIPLPKFQGKLYYAISDSGCKYREYS-KGDPW---PKGLKQMDCFFGPRCTAIGKRKIVLQV------ 1EKG Chain:A ((90-208)) ----LDETTYERLA--EETLDSLAEFFEDLADKPYTFEDYDVSFGS---GVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELL-AAELTKALKTKLDLSSLAYSGK

General information: TITO was launched using: RESULT: Template: 1EKG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 411 -27137 -66.03 -248.96 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -66.03 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.222

(partial model without unconserved sides chains): PDB file : Tito_1EKG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EKG-query.scw PDB file : Tito_Scwrl_1EKG.pdb: