Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------KKEKDADTDPDTKMCDFVIKKSGKKI-TSGSVWPGATENFMVDGQWPVVSATQQCRITMSGLSDIDQYTITLCDKKEDETVSSDGRNTTVISSHGEWC------------------------------------------------------------------------------ 1TFJ Chain:A ((16-219)) EHLLRPLPADKQIETGPFLEAVSHLPPFFDCLGSPVFTPIKADISGNITKIKAVYDTNPTKFRTLQNIL--EVEKEMYGAEWPKVGATLALMWLKRGLRFIQVFLQSICDGERDEN------HPNLIRVNATKAYEMALKKYHGWIVQKIFQAALYAAPYKSDFLKALSKGQNVTEEECLEKVRLFLVNYTATIDVIYEMYTRMNAELNYKV

General information: TITO was launched using: RESULT: Template: 1TFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 265 -351 -1.32 -3.94 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -1.32 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.008

(partial model without unconserved sides chains): PDB file : Tito_1TFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TFJ-query.scw PDB file : Tito_Scwrl_1TFJ.pdb: