Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence NCEITIL-QGESRIGFVNVPSSGKVRENI-RNWWYTISCDEDCNPKIS-------GFTYTVEKAC 2MM2 Chain:A ((1-65)) NCTANILNINEVVIATGCVPAGGNLIIRVGSDHSYLIRATVSCGLSLNPSQSFINGESLASGGRC

General information: TITO was launched using: RESULT: Template: 2MM2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 207 -14266 -68.92 -254.74 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -68.92 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_2MM2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MM2-query.scw PDB file : Tito_Scwrl_2MM2.pdb: