Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------CTYSVYDSIGKMVVEDKDLPTNRRDYYTIGKKKYTFYFKE-NCDFDYSKPPLPRCWHVSGIPKGLYDQVQGMEQPCPAQ----------------------------------------------------------------------------------------------------------------------------------------------------- 1KCF Chain:A ((3-256)) TVKLSFLQHICKLTGLSRSGRKDELLRRIVDSPIYPTSRVLGIDLGIKNFSYCFASQNED---SKVII-HNWSVENLTEKNGLDIQWTEDFQPSSMADLSIQLFNTLHEKFNPHVILMERQRYRSGIATIPEWTLRVNMLESMLYALHYAEKRNYPFLLSLSPKSTYSYWASVLNKKSRVQMVKELIDGQKILFENEEALYKWNNGSRVEFKKDDMADSALIASGWMRWQAQLKHYRNFCKQFL

General information: TITO was launched using: RESULT: Template: 1KCF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 178 9102 51.13 122.99 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 51.13 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_1KCF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KCF-query.scw PDB file : Tito_Scwrl_1KCF.pdb: