Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WTGKSEASNRPPPGRRPEETT---WFTSDPSYACGVKIFRPGS--EGSSGLAEITHLNFHEKIKVLKGQYECEVGPSCSTLRCTMPRGWRLDIFGKSFPTRRTTYFAENGNPEYNTL--ASEANIPLYDCRVVIRFPPTGGRDTGPLAEATHFNLGEVVGVLGGRHTCQAQGQCANVNCNVPKGYTYYSLTGGPFQTKEKSHTFQSDA- 1F2Q Chain:A ((4-174)) ---KPKVSLNPPWNRIFKGENVTLTCNGNNF---STKWFHNGSLSEETNSSLNIVNAKFEDS-----GEYKCQHQQVNESEPVYLEV--FSDWLLLQAS---AEVVMEGQPL---FLRCHGWRNWDVY--KVIYYKDGEALKYWYENHNISITNATVE---DSGTYYCTGKVW------------------QLDYESEPLNITVIKAPR

General information: TITO was launched using: RESULT: Template: 1F2Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 734 26676 36.34 167.77 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 36.34 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.104

(partial model without unconserved sides chains): PDB file : Tito_1F2Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F2Q-query.scw PDB file : Tito_Scwrl_1F2Q.pdb: