Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --STSTKVKLENRKEPA-------LCHVVIIKPAQIRHPLDTWVETFAWVKAGKVVEWHSPKYGSVKVKTDE---DD------CHLTFLVNELING--YTCQLSR--------------- 1FNG Chain:A ((82-182)) DANVAPEVTVLSRSPVNLGEPNILICFIDKFSPPVVN------VT---WLRNGRPV-----TEGVSE--TVFLPRDDHLFRKFHYLTFLPST---DDFYDCEVDHWGLEEPLRKHWEFEE

General information: TITO was launched using: RESULT: Template: 1FNG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 176 729 4.14 11.04 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 4.14 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.623

(partial model without unconserved sides chains): PDB file : Tito_1FNG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FNG-query.scw PDB file : Tito_Scwrl_1FNG.pdb: