Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GCEVLISKKSGGDGPVKSSCIPKSGSKVIVI-NGKTVTVSADGSCKFS---SKDLDPSLAMKFEGDCIGI 2MM2 Chain:A ((1-65)) NCTANILNINEV--VIATGCVPAGGNLIIRVGSDHSYLIRATVSCGLSLNPSQSFINGESLASGGRC---

General information: TITO was launched using: RESULT: Template: 2MM2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 259 -10300 -39.77 -168.84 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -39.77 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_2MM2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MM2-query.scw PDB file : Tito_Scwrl_2MM2.pdb: