Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------PEHCFVRILKYEIPIHGDGYLVGSDVNWWVGNQKVD----------FHINNDCTIQRRGG---TQEKKPYRLDSISTLGEDLPKGFDFILFRESKIDEFLAQGGVTKRILGSMIL-- 4F55 Chain:A ((1-128)) GGGRTNVPNGYSQNDIQLMANAVYGES--RGEPYLGQVAVAAVILNRVTSASFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTRPQIKKI-GKHIFCK

General information: TITO was launched using: RESULT: Template: 4F55.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 418 4128 9.87 41.69 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 9.87 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_4F55.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F55-query.scw PDB file : Tito_Scwrl_4F55.pdb: