Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------RPSSPEEHYQEKMYPLSDMDSEQTINGCLV-------------------------CIFAGSFKP--SRTLGDCLGAKCIKFHQSDYITGIRVSVGETCNDVRLGRTLTSWSVRVNPS---EC------------------ 1V7L Chain:A ((1-162)) MITTGKVWKFGDDISTDEITPGRYNLTKDPKELAKIAFIEVRPDFARNVRPGDVVVAGKNFGIGSSRESAALALKALGIAGVIAESFGRIFYRNAIN-IGIPLLLGKTEGLKDGDLVTVNWETGEVRKGDEILM--FEPLEDFLLEIVREGGILEYIRRRGDLCI

General information: TITO was launched using: RESULT: Template: 1V7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 401 23272 58.03 261.48 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 58.03 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_1V7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V7L-query.scw PDB file : Tito_Scwrl_1V7L.pdb: