Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KITV--CQVE---------LW------GADSTKPSDTAWVSPGGIARFTVKFGRHSL----FLYAKTSGDCTPSPVLEGPSRWGTHTIKGSLQEKQKGTVLIQEV-SMKEATRGHP--------------------- 2JOZ Chain:A ((1-135)) MIMVSGCQQQKEETPFYYGTWDEGRAPGPTDGVKSATVTFTEDEVVETEVMEGRGEVQLPFMAYKVISQSTDGSIEIQYLGPY--YPLKSTLKRGENGTLIWEQNGQRKTMTRIESKTGREEKDEKSKSLEHHHHHH

General information: TITO was launched using: RESULT: Template: 2JOZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 265 8697 32.82 94.53 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 32.82 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.200

(partial model without unconserved sides chains): PDB file : Tito_2JOZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JOZ-query.scw PDB file : Tito_Scwrl_2JOZ.pdb: