Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSSGPGPRFLLLLPLLLPPAASASDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEEWRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTELLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFL--HNNEVF--HEPHIADSWPQLQ-----DHFSAVKLVGPEEA---------LSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHG-----------KLWSNFWGAL----------SP-----DEYYA-----RSEDYVELVQRKRVGVWNVPYI-SQAYVIRGDTLRME--LPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP 3ZF8 Chain:A ((22-302)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VNKEHILILTPMQT---FHQQYWDNLLQLNYPRELIELGFITPRTATGDLALKKLENAIKKVQTDKKTQRFSKITILRQNS-ALDVQKERRAAMALARNELLFSTIG-PHTSWVLWLNADIIETPPSLIQDMTKHNKAILAANIYQRFYDEEKKQPSIRPYDFNNWQESDTGLEIASQMGDDEIIVEGYAEIATYRPLMAHFYDANGVPGEEMALDGVGGGCTLVKAEVHRDGAMFPNF--P-FYHLIETEGFAKMAKRLNYDVFGLPNYLVYHIEE-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZF8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 854 -26919 -31.52 -124.05 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -31.52 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_3ZF8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZF8-query.scw PDB file : Tito_Scwrl_3ZF8.pdb: