Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHY 2MRJ Chain:A ((149-231)) WYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHY

General information: TITO was launched using: RESULT: Template: 2MRJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 285 -26206 -91.95 -315.73 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -91.95 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.776

(partial model without unconserved sides chains): PDB file : Tito_2MRJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MRJ-query.scw PDB file : Tito_Scwrl_2MRJ.pdb: