Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFHGAISREDAENLLESQPLGSFLIRVSHSHVGYTLSYKAQSSCCHFMVKLLDDGTFMIPGEKVAHTSLDALVTFH 2CS0 Chain:A ((18-93)) WFHGAISREDAENLLESQPLGSFLIRVSHSHVGYTLSYKAQSSCCHFMVKLLDDGTFMIPGEKVAHTSLDALVTFH

General information: TITO was launched using: RESULT: Template: 2CS0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 279 -55320 -198.28 -727.89 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -198.28 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_2CS0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CS0-query.scw PDB file : Tito_Scwrl_2CS0.pdb: