Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGVETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNLYPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSANGETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSYTDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKES-PADWQVVTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQAK---DRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFTGVRHFRH 4EHI Chain:B ((23-534)) NAMRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGIKVIEVSDFTKS-------VKTLHPKIHGGILHKRSDENHIKQ--ENEILGIDLVCVNLY-----------DFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLKK-GQNDENFRLNLMIKAYEHTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEF-DAF--FSANFKALKG-EASFNNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVAINGTLDEALANKINEIYVEVIIAANVDEKALAVFEGK-KRIKIFTQESPFLIRSFDKYDFKHIDGGFVYQNSDEVGEDELKNAKLMSQREASKEELKDLEIAMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKAAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEPGGSIRDDEVVKAADEYGMALYFTGVRHFLH

General information: TITO was launched using: RESULT: Template: 4EHI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2445 -179627 -73.47 -367.34 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -73.47 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4EHI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EHI-query.scw PDB file : Tito_Scwrl_4EHI.pdb: