Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKFSMEKENRGKNMAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSITVVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFS------------------DIELFNAKDDKVTVLSIERGNKLVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFKLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSETYKRSDYYKIKFISCDRDH 4ZS8 Chain:A ((25-247)) -----------------PKYYRLKKHLLD--MTRTQTPGTPVPPERTLAAEFDTSRTTVRQALQELVVEGRLERIQGKGTFVAKPKVSQALQLTSYTEDMRAQGLEPTSQLLDIGYITADDRLAGLLDITAGGRVL--------------RIERLRMANGEPMAIETTHLSAKRFPALRRSLVKYTSLYTALAEVYDVHLAEA--EETIETSLATPREAG-LLGTDVGLPMLMLSRHSQ-DRTGQPVEWVRSVYRGDRYK----------

General information: TITO was launched using: RESULT: Template: 4ZS8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 759 -10530 -13.87 -51.36 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -13.87 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_4ZS8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZS8-query.scw PDB file : Tito_Scwrl_4ZS8.pdb: