Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAYTERVFGNV-EGEDVLAYRFETD-GGYQLEVMTYGATILRYVAPDKAGNFANVILGFDDFDSYVG-NSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNASNCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEISYKV-TTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAK-TPEANRDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHSFALPAGH----DNAGFLYDQNSGRFLLFKTEAPCFVVYTANFVDES-VIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTFTSKTRYELVVK 4RNL Chain:A ((7-342)) --QVSREPFGTLDDGTRVDRWTLESGPAGLRVRVLTYGGIVQTVEAPDRDGMRGQLALGFADLASYAAHGGSYFGALVGRYANRIAGASFVLDGRTDALTPNNGRHSLHGGPGGFSRVVWDAREVD-GGVQLHRVSPDGEEGFPGALDVRVTYTLSA-GALRIVSCATT-DAPTVVNLTNHTYLNLGGDGSGSAAGHELRLAASRYTPVDGTGIPVPGAPAEVTGTRFDFRAARAVA-------------GAYDHNFALDGGVREAPRTVAELYDPRSGRALALATTEPGLQLYTADHLDGTLTGTSGVPYGPAAGLALETQHFPDSPNRPDFPSTVLRPGESYRSETVYAFSV-

General information: TITO was launched using: RESULT: Template: 4RNL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2026 37474 18.50 114.95 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 18.50 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4RNL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RNL-query.scw PDB file : Tito_Scwrl_4RNL.pdb: