Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNSKYITCLKRSEGQLRGIQKMIEGDRDCADIVTQLTAVRSSVERVIEMIITENLTECINQPLDDSE-AQKERLEKAIRYLIKRK 4UIG Chain:A ((50-133)) -QKAEHLKRLRRIEGQIRGLQRMVDEDVYCIDILTQVSASTKALQSFALQLLEEHLRHCVADAALKGGTEIDAKVEEATKAIGRL-

General information: TITO was launched using: RESULT: Template: 4UIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 121 -14954 -123.59 -180.17 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -123.59 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_4UIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UIG-query.scw PDB file : Tito_Scwrl_4UIG.pdb: