Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAWIRQLLYKIKQSSGAVFLCFVLKNLKFVFVSFRKIRYTVCKRFQRSPVMAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQPLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYTGTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLAQFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLVDKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEIQPILGQDFKTLAQEK 5G04 Chain:F ((480-573)) -----------------------------------------------------------------------NCKEAINILSHLPSHHYNTGWVLCQIGR-AYFELSEYMQAERIF--SEVRRIENYRVEGMEIYSTTLWHLQK---DVALSVLSKDLTDMDKNSPEAWCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5G04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 351 -31579 -89.97 -335.95 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain F : 0.55 3D Compatibility (PKB) : -89.97 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.128

(partial model without unconserved sides chains): PDB file : Tito_5G04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G04-query.scw PDB file : Tito_Scwrl_5G04.pdb: