Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRTEYLTQLELYLKKLPEADRIEAMDYFRE-LFDDAGIEGEEELIASLGTPKEAAHEVL---SNLLDKKINEAPAQKNNRQILHIALLA-LLAAPIGIPLGIAILVTLFAI---LVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGASSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA 5XAM Chain:A ((210-319)) --------------------DQIQSVATINQRLFRDIQISGN-------FTPEEASQLALVLKSGALPIKIVTAAERSIGPSLGADAIRSGAIAALVGIGLVFVMLFAYYGLWFGLVGALG--LLFSSIIILGILGGF-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5XAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 92 -9545 -103.75 -94.50 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -103.75 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_5XAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5XAM-query.scw PDB file : Tito_Scwrl_5XAM.pdb: