Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMERNLEVMKASVQLGLN-ENKSRSGLTG-GDAAKLDHYIENG-----KTLSDYTILSAARNAIAVNEHNAKMGLVCATPTAGSAGCLPSVLTAAIEKL-DLSHEQQLDFLFAAGAFGLVIANNASISGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVKRNAMGASFAFIAADMALAGIE-SKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGRRLQKEIFGE 4RQO Chain:B ((190-473)) PFATATELLKLCK-KHHLTIAELMLVNEKT--WRSTAEIHKGILDIAKVMDDCINNGCKHDGVLPGGLNLKRRAPDLYRKLIEQKGVKSVFEQSDIMNHLNLYAMAVNEENAAGGRIVTAPTNGAAGIIPAVLKYCQQAHDRMSNEDIYTYFLTAAAIGILYKK----SGAEVGCQGEVGVASSMAAAGLTAVLGGTIEQVENAAEIAMEHHLGMTCDPVLGLVQIPCIERNAMGAVKAVNATRMALIGDGQHQISLDKVIKTMKQTGMDMQSIYKETSMGGLAVNL------------

General information: TITO was launched using: RESULT: Template: 4RQO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1458 -70358 -48.26 -259.62 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -48.26 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_4RQO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RQO-query.scw PDB file : Tito_Scwrl_4RQO.pdb: