Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKEKVILAYSGGLDTSVAITWLKKD--YDVVAVCMDVGEG-KDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKENGVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQKLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK 4XFJ Chain:B ((10-405)) --SERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGGEDMEVVRQRALDCGAVESIVIDARDEFANDYCVPAIQSNALYMDRYPLVSALSRPLIVKHLVKAAREHGGTIVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWTREKAIAFAEENNIPINVTKRSPFSIDQNVWGRAVETGFLEHLWNAPTKDVYSYTEDPTVNWSTPDEVIVGFEQGVPVSIDGRSVTPLQAIEELNRRGGEQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHTELEHVTLERELGRFKRITDQKWGELVYDGLWFSPLKTALESFVAKTQEHVTGEIRMVLHGGHIAVNGRRSPKSLYDFNLATYDEGDTFDQSAAKGFVQIHGLSSSISARRDL---

General information: TITO was launched using: RESULT: Template: 4XFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2036 -38490 -18.90 -97.94 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -18.90 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_4XFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XFJ-query.scw PDB file : Tito_Scwrl_4XFJ.pdb: