Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MGWIHICDS------------KMSNVDKIRKIHIIVCWMYIFLSFNP----IISNTEYFLLIFLAFIYSIVSLPLYSVKNKIVSICLA----INSILLMSFPILINKFFPESFLTYTVLISVFITELIIFHLIGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVD----NHVLTLIFLIKICVDTTIKFL----FIRLFDTS-TLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSVLLLFSNFSVPFIKE--KEFELFKNSK-- 5IPX Chain:A ((32-322)) HLFNHLFRYHYPSWDQILQELDTLSVATLNPDCHVPALNVEKTLYLAKTIQILVQHRQSEPYLVPAARANLAYSLQQLYKLGNDKIRGVINGMLPLVDAGCIGFERELIKGLPRVLTLQYPHT---------APCTEWCLSHFVGAS--GRLRSEVRDILTTHNG-TCAPSFEWMASVVKKFFLVETVIYEDFQDTDFNVQLNLCFFWTAVVQMYQRCIYEQKLVHIISTSLTLLKSTARSFFAWYDLYRPNLGSAALVKYTEHLIRALT--PDCSDVELGELCSHLHHCKHALFS

General information: TITO was launched using: RESULT: Template: 5IPX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 892 -45638 -51.16 -191.75 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -51.16 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_5IPX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IPX-query.scw PDB file : Tito_Scwrl_5IPX.pdb: