Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEIKRIQQQPDLAQDIYAVMAAVYPVSPWTLEQIQADLSQDQTWYALAYDGAEVIGFLTVQETLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKE------IFLEVRQSNQRAQAFYKKEKMAVIAERKAYY--HDPVEDAIIMKREIDEG 5ISV Chain:A ((26-142)) ----------------------------PWSEKTFASNQGERYLNFQLTQNG-KMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIM-------

General information: TITO was launched using: RESULT: Template: 5ISV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 342 -22399 -65.49 -205.50 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -65.49 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.307

(partial model without unconserved sides chains): PDB file : Tito_5ISV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ISV-query.scw PDB file : Tito_Scwrl_5ISV.pdb: