Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSMLSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEVDQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCNTMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFEAELER-MLRPYDVFEKVSWQFLKKMSVFLQTKGSN-QKEIERFIQSLQVLENPQLTSLFELRFQQYKELID 4YV9 Chain:D ((4-273)) -----ELGKTLRRLRQGKQVSISSLADEHLSKSQISRFERGESEISCSRLLNLLDKLNITIDEFVSTHS----THFFTLLSRVRKYYAEKNVAKLLKLLEDYAHK--DYE-S----TMIKAILSSIEPTVEPSEEEVTRLTDYLFSVEQWGYYEIILLGNCSRFINYNTLFLLTKEMVTSFAYSEQNKTNKTLVTQLSINCLIISIDYSYFDHSHYLIEKIEFLLRDELNFYEKTVFLYVHGYYKLKQ----SGKDDMRQALQIFKYLGEDALYYSYKEHYRKE-----

General information: TITO was launched using: RESULT: Template: 4YV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 992 -116937 -117.88 -446.32 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : -117.88 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_4YV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YV9-query.scw PDB file : Tito_Scwrl_4YV9.pdb: