Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYSFTKGAIILSGGSIMKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVAGVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVV-VA-IVFLVRKNLQYANYTYVGYVLLAIIGSIYGYVGLQDA-VQLVQDESMRLTVSI-GSKAVSIFYIVINVLFLALVFYKMWRQQKALAEEEETEELT- 5TPM Chain:A ((1-155)) -----------SNADLLETRHALEGIAAYYAALRSTDEDKERIREL--HHAIELAQQS--GDLDAESNAVLQYQIAVTEAAHN------VVLLHLLRCMEPMLAQNVRQNFELL-YS-------------------RREMLPLVSSHRTRIFEAIMAGK--PEEAREASHRHLAFIEEILLDRSREESRRERSLRRLE

General information: TITO was launched using: RESULT: Template: 5TPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 556 -24838 -44.67 -165.59 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -44.67 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_5TPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TPM-query.scw PDB file : Tito_Scwrl_5TPM.pdb: