Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRFLKSINKGVQEVRLILYEKQGEKFMKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIKGKIDDQVYLVEIGKIQRFYIENRKVLAE--------TASQTYSIDL-------RLYQVLKLLPSNFIQISQSEIINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL 1R9Z Chain:A ((217-307)) -----------------------------------------------------------------------------VHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVA-------------------NPLVNIHLQGRFDTYPKRRGITRVKEMLE-

General information: TITO was launched using: RESULT: Template: 1R9Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 265 -17735 -66.92 -233.35 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -66.92 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_1R9Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R9Z-query.scw PDB file : Tito_Scwrl_1R9Z.pdb: