Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAMLALGYKATELKK-----IKKFFEGTTDTAENYIKSALKMLVK 2ZTD Chain:B ((17-210)) MIASVRGEVLEVALDHVVIEAAGVGYRVNATPATLATLRQGTEARLITAMIVREDSMTLYGFPDGETRDLFLTLLSVSGVGPRLAMAALAVHDAPALRQVLADGNVAALTRVPGIGKRGAERMVLELRDKV-----------------VRSPV---VEALVGLGFAAKQAEEATDTVLAANHDATTSSA---LRSALSLLGK

General information: TITO was launched using: RESULT: Template: 2ZTD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 746 -112456 -150.75 -646.30 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -150.75 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.670

(partial model without unconserved sides chains): PDB file : Tito_2ZTD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZTD-query.scw PDB file : Tito_Scwrl_2ZTD.pdb: