Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALDSTTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLTIEEPWKA-----SEVLSGAKPILIVEGMSVGFLP--KELFEKTICFYTDEETELKRRLARDTTVRNRDASFILASH-QMRREQYLRYYKETESKADILVDQSEDKFDVKRT 3ASZ Chain:B ((4-190)) ----------------PKPFVIGIAGGTASGKTTLAQALARTLGE-RVALLPMDHYYKDLGHLPL--EER-LRVNYDHPDAFDLALYLEHAQALLRGLPVEMPVYDFRAYTRSPRRTPVRPAPVVILEGILVLYPKELRDLMDLKVFVDADADERFIRRLKRDVLERGRSLEGVVAQYLEQVKPMHLHFVEPTKRYADVIVPRGGQN------

General information: TITO was launched using: RESULT: Template: 3ASZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 842 -23415 -27.81 -130.81 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -27.81 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_3ASZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ASZ-query.scw PDB file : Tito_Scwrl_3ASZ.pdb: