Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------MTWFE--EIFEEYKFEKGEGQCPRLLCFLVASFVKRVIIDCRIKGGIYERDSRIIY------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 4ZLA Chain:A ((7-502)) MLKIKLEKTTFENAKAECSLVFIINKDFSHAWVKNKELLETFKYE-GEG----------------VFLD---------QENKILYAGVKEDDVHLLRESACLAVRTLKKLAFKSVKVGVYTCGAHNALLENLKALFLGLKLGLYEYDTFKSNKKESVLKEAIVALELHKSLEKSAKEALKYAEIMTESLNIVKDLVNTPPMIGTPVYMAEVAQKVAKENHLEIHVHDEKFLEEKKMNAFLAVNKASLSVNPPRLIHLVYKPKKAKKKIALVGKGLTYDCGGLSLKPADYMVTMKADKGGGSAVIGLLNALAKLGVEAEVHGIIGATENMIGPAAYKPDDILISKEGKSIEVRNTDAEGRLVLADCLSYAQDLNPDVIVDFATLTGACVVGLGEFTSAIMGHNEELKNLFETSGLESGELLAKLPFNRHLKKLIESKIADVCNISSSRYGGAITAGLFLNEFIRDEFKDKWLHIDIAGPAYVEKEWDVNSFGASGAGVRACTAFVEELLKKA

General information: TITO was launched using: RESULT: Template: 4ZLA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 15 2058 137.17 73.48 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : 137.17 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.727

(partial model without unconserved sides chains): PDB file : Tito_4ZLA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZLA-query.scw PDB file : Tito_Scwrl_4ZLA.pdb: