Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEAVHTEKQWERSATELPPFIIKRLPVRLTFDNNYFNDRYQVIPIGGYNVIIENILKDVEVGLGVDCFDYREELEASANKVAFTGMIDQYFDYKHGELEYRSLRFKHEVLDEENHQGNAVVNYTDREIPYTRIIEHKKFEYGLQPKTVITHEYPADWKRGDEPYYPINDEKNNAIFSKYHEEASQNDKVIFCGRLADYKY 1I8T Chain:B ((151-346)) -----YTEKQWGRSAKELPAFIIKRIPVRFTFDNNYFSDRYQGIPVGGYTKLIEKMLEGVDVKLGIDFLKDKDSLASKAHRIIYTGPIDQYFDYRFGALEYRSLKFETERHEFPNFQGNAVINFTDANVPYTRIIEHKHFDYVETKHTVVTKEYPLEWKVGDEPYYPVNDNKNMELFKKYRELASREDKVIFGGRLAEYKY

General information: TITO was launched using: RESULT: Template: 1I8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 589 -17920 -30.42 -91.43 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -30.42 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_1I8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I8T-query.scw PDB file : Tito_Scwrl_1I8T.pdb: