Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MSLLETAKRHQLNSE--KYLSYLLECL----PNEETLVNKEVLEAYLPWTKVVQEKCK 2MH3 Chain:A ((26-95)) MKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSRHSKLEKADILEMTVKHLRNLQRAQ-

General information: TITO was launched using: RESULT: Template: 2MH3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 28 2016 72.00 39.53 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 72.00 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.728

(partial model without unconserved sides chains): PDB file : Tito_2MH3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MH3-query.scw PDB file : Tito_Scwrl_2MH3.pdb: