Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYYTGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFEEVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILRKIVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKIFLTTMIGDVEVVRG 4QRK Chain:A ((119-220)) ---------------------------------------------------------------------------------------------------------------------------SKIN-LKELNVGMGVGKVDLDISGNY--KNNVKVNIEGGVGEATVYLPKSIGVKIKAEKGVGAVNANGFI-VE---GENIYKNSQYGKSKNSIEVNIEAGVGAINIKQK

General information: TITO was launched using: RESULT: Template: 4QRK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 563 -49491 -87.90 -485.20 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -87.90 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_4QRK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QRK-query.scw PDB file : Tito_Scwrl_4QRK.pdb: