Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEVDTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA 2F2A Chain:C ((3-94)) -KVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDSADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTI-------

General information: TITO was launched using: RESULT: Template: 2F2A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 106 -12210 -115.18 -132.71 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -115.18 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.713

(partial model without unconserved sides chains): PDB file : Tito_2F2A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F2A-query.scw PDB file : Tito_Scwrl_2F2A.pdb: