Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKEMLEAFRILEEDKRIKKEDIIDAVVESLRSAYRRRYGQSDSVAIDFNEKTGDFTVYTVREVVDEAFDSRLEISLKDALAINSAYELGDKIKFEEAPAEFGRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQLSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDGTVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDRMVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDESDSKRALVVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE 4MTN Chain:A ((5-325)) -GNEVLRIVDSIHRDKSIDKEIVFEGVEQAILSAARKHFGEEEVIEVHIDRTSGQPMVKTNG----------REID--------R-DELGD---------ILGRISAQT-KQVMIQKIREAERDTLFDEYAQLRGQIVSGTVTRNEGSAITVNIGKAEAILPRSEMIPGESHRPNERIRAVVLEVKKMGPRVRVVLSRAHPDFVRRLLELEIPEVNERIIEIRSLAREAGYRTKVAVSCADSNIDPVGACVGVRGARIRNVGEELGG--------------ERIEVVRWNDSLQVLVPNAMQPSEVEDVILCP-MLGRVLVLVRDDQLSLAIGKRGQNVRLASKLVGWDIDVMTREELDQQLDQA-------------

General information: TITO was launched using: RESULT: Template: 4MTN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1438 -164245 -114.22 -513.26 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -114.22 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4MTN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MTN-query.scw PDB file : Tito_Scwrl_4MTN.pdb: