Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSQDNGMMESFFGILKFMAMRKHLNHLINWNKLL 1VSM Chain:A ((58-169)) LQIWQTDFTLEPRM--APRSWLAVTVDTASSAIVVTQHGRV-TSVAAQHHWATAIAVL-GRPKAIKTDNGSCFTSKSTREWLARWGIAHTTGIPGNSQGQAMVERANRLLKDKIRV-------------

General information: TITO was launched using: RESULT: Template: 1VSM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 508 -34837 -68.58 -311.04 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -68.58 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_1VSM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VSM-query.scw PDB file : Tito_Scwrl_1VSM.pdb: