Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRQTPSLSFEVFPPTPAVGNDNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKVDAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDIAGVHLYTMNNADTAKYIHQATHALFNHQSLG 2FMO Chain:C ((24-286)) ------NVSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANS-E-RDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGDL---------EMYASDLVTLLKEVA-DFDISVAAYPEVHPEAKSAQADLLNLKRKVDAGVNRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKFADMT-NVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAI--------------

General information: TITO was launched using: RESULT: Template: 2FMO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1275 -180339 -141.44 -707.21 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -141.44 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_2FMO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FMO-query.scw PDB file : Tito_Scwrl_2FMO.pdb: