Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVFLITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVKESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQSMGVDVIVTDHHSMPETLPDAYAIVHPEH-PDADYPFKYLAGCGVAFKLACALLEEV----QVELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEETVGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEAKTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAVDIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNLDEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISKGEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFNIRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYF-KNDIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYK-------LKDLAAYLNIQQILLVKMIQVFEELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE 5F56 Chain:A ((2-704)) ------SRPAHWLLAPPASRDALLATMREWQVSPPVAQVLCGRDLR-----TELLALPLELTPN----PALREAARHIVAAVREGKRIRIHGDYDADGVSATATLVLGLRAIGANVHGFIPHRLNEGYGIHPDRVPEHAAA--ADLVVTVDCGVSNLDEVKSLLATGTEVVVTDHHAPGENFPEC-LVVHPHLTPDYDPDRHNLTGAGVAYHLLWAVYEELGRPEPRALLPLATLGTVADVAPLLGENRALVRAGLAEMARTELPGLRALMNEKRV--RQPTARDVAFILAPRINAAGRMGEADRALELLTTPSDHEAKSLAAYLEIRNQERRKIQDDMFAQALQLADPNDPALVLTHDDWHAGVMGIVASKLVETFNRPVYIVA----QGKGSVRSTPGISAVQGLRESRDLLGRFGGHPGAAGFSLDPQNFGALRERIHGYVRQFPTP---VPAVRLDAPLPVAALTPELLSELSILEPFGEGNPRPLWHLRG-PLTDTRLVGKQGDVLQFRFG----GVKGMKYSERD-----DAAGERDVAAELALNEWKGRTSLELHAAALRP--LAPLALAGTEEGLPT-LPRLN-PREAMTFLKTGAAAYAE-----QGVATYLRDNVPGLTLLDTNAPHPGGDLILYGLPPESA-LRRWLHEAQEQGGRVAFALGPKTLAELDAALTLAKLLPDSHTEAAQEAAADAYRSWQWAHHYRVLNDAGWSASVYAMLGLPVPAA-------LPKAAEALALAA

General information: TITO was launched using: RESULT: Template: 5F56.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3971 -8629 -2.17 -12.51 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -2.17 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_5F56.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F56-query.scw PDB file : Tito_Scwrl_5F56.pdb: