Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDFRFLQRDLTLDLEPIFDWCPQVDVLC-----NTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVELTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGIQVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAMQGQILTIDGGWSLK 1O5I Chain:B ((20-245)) --KGVLVLAASRGIGRAVADVLSQEGAEVTICARNEELLKRSGHRYVVCDLRKDLDLLFEKVKEVDILVLNAGGPKAGFFD------ELTNEDFKEAIDSLFLNMIKIVRNYLPAMKEKGWGRIVAITSFSVISPIENLYTSNSARMALTGFLKTLSFEVAPYGITVNCVAPGWTETE-RVKELLSEEKKKQVESQIPMRRMAKPEEIASVVAFLCSEKASYLTGQTIVVDGGLS--

General information: TITO was launched using: RESULT: Template: 1O5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1107 -68029 -61.45 -307.82 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -61.45 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_1O5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O5I-query.scw PDB file : Tito_Scwrl_1O5I.pdb: